Anti-Proliferative Activity of Aescin on Human Oral Carcinoma (KB) Cells and Human Laryngeal Carcinoma (Hep-2) Cells- Via Cell Sensitivity Assays

Abstract

            The incidence of Head and Neck malignancy is expanding around the world. The critical risk factor for these tumors incorporates smoking, liquor, tobacco intake, and viral infection. Natural agents have frequently shown restorative properties with helpfulness in treating different human degenerative diseases. Aescin is the most prominent constituent of Aesculus hippocastanum L. (Hippocastanaceae) seeds has been conventionally utilized as a therapeutic herb. We determine the anticancer effects of aescin on human oral carcinoma (KB) cells and human laryngeal carcinoma (HEp-2) cells by cell sensitivity assays. To vindicate the impact of aescin compared with positive control cisplatin on anti-proliferative activity in human oral carcinoma and human laryngeal carcinoma cells were investigated by cell sensitivity assays (MTT assay, Tryphan blue dye exclusion method, Alamar Blue assay, and Hexosaminidase assay). The 24-hour treatment of KB cells and HEp-2 cells with different concentrations of aescin suppressed proliferation and induced morphological changes consistent in these cells. Exposure of both cell lines to aescin resulted in a marked increase in loss of cell proliferation and induces cytotoxicity in KB and HEp-2 cells. Taken together, our results demonstrate that aescin modulate cell growth and differentiation in KB and HEp-2 cells by initiate cytotoxicity implying that aescin might be developed as a potential chemotherapeutic agent for the treatment of head and neck cancer.