Influence of Capparis Spinosa Crude Extract on the Amylase Activity in Vitro

Abstract

The present study is designed to Study the effect of some extracted chemical components from C. spinosa leaves and fruits on - amylase activity in vitro.

Subjects:In the method extracted the active substances in the biology of the leaves and fruits of this plant using two solvents, water and ethanol . The biological compounds were estimated by means of the chemical reagents detection method and the optical density spectrum method under the flesh Behind.The effect of these extracts on inhibiting amylase enzyme has also been studied in different concentrations ( 25 , 12.5 , 6.25 , 3.125 , 1.5625 mg / L ).

 Results:There is no significant difference between the product in ethanol extraction and the extraction product in distilled water. As the result showed that both the leaves and fruits have an effect on the enzyme activity , since they are all inhibited the enzyme function . This result can be useful for referring  functional effect on the digestion of carbohydrates , which may be has a significant in the treatment of diabetes.

Conclusion:There is not that much difference in the effect of the extracts with different solvents because both solvents used are polar , it is noted that leaf extracts and fruits have effect in inhibiting the alpha amylase enzyme.