IL-6 Predict Disease Severity and Potential Therapeutic Target in COVID-19 Patient.
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Abstract
Background:Abnormal immunological features were well-known in patients with COVID-19 is higher serum concentrations of inflammatory mediators. In deceased patients, serum level of interleukin 2, IL-6, IL-8, and TNF a show significant higher concentration than in improved patients. specifying the need for IL-6 detection for initial expectation of severity. Therapeutic management aimed at correcting excessive and aberrant host immune responses are postulated to be successful.
Thus, the aim of present works is to find out the distribution of IL-6 serum concentration among mild, and sever cases of patients with COVID-19 infection in Iraqi populations, and to find out whether SNP of IL-6 rs1800795 174 (C/G) genotype could effect on disease severity.
Patients and Methods: The case control study was established on the basis of three groups. 1st group was include 15 healthy volunteers which considered as a control groups, 2nd group include 20 mild cases of patients with SARS-CoV-2 infection, and 3rd group include 20 sever ill patients with SARS-CoV-2 infection, 5 ml of Blood samples were collected by venipuncture from 40 patients and 15 healthy controls, by disposable syringe under aseptic technique, about 2 ml of venous blood were drawn by in ethylinditetracitic acid (EDTA) tube and stored at 4℃ for DNA extraction for detection of IL-6 gene polymorphism by ARMS-PCR. And Three milliliters of blood were put in gel tube and allowed to clot then the serum was separated by centrifugation 1500 rpm for 5 minute. Then serum has been separated in Eppendorf tube for IL6 detection by ELISA test.
Results: Present study demonstrated that, IL-6 CC genotype was more frequent in severely ill patients with COVID-19 group than in healthy control group, in a significant manner (p = 0.014). Therefore, C/C genotype can be regarded as a risk factor. Regarding allele analysis, allele C was more frequent in severe group than in healthy control group, in a significant manner (p = 0.018). Therefore, C allele can be regarded as a risk factor.
Conclusions: In conclusion, We identified a strong correlation between the case severity of COVID-19 and frequency C/C genotype of the rs1800795174 SNP gene, in addition present data recommended to consider IL6 cytokines as akey factor to realize the therapy response in COVID-19 infected peoples.