Effectiveness of Edible Film Chitosan from Waste White Shrimp (Litopenaeusvannamei) in Reducingcolonization of Porphyromonas Gingivalis Bacteria: In Vitro Research

Abstract

Background: Chitosan is a biopolymer that usually extracted from the shells of crustaceans such as crabs, shrimp, and lobster. The advantages of chitosan as an antimicrobial are abundant availability, low production costs, excellent biodegradability, and biocompatibility. Chitosan can show antibacterial activity against bacteria that cause periodontitis, one of which is Porphyromonasgingivalis. Objective: This research aims to observe and identify the effectiveness of chitosan edible films in inhibiting the growth of Porphyromonasgingivalis bacteria. Method: This study uses a laboratory experimental research type using the Post-test only with control group research design. In this study, six repetitions were carried out in 8 treatments with six repetitions each, namely chitosan extract with concentrations of 1%, 2%, and 3%, edible chitosan film 1%, 2%, and 3%, positive control (edible film metronidazole), as well as the negative control. The measuring instrument used in this study is the calipers in millimeters (mm). Results: Least Significance Different (LSD) result shows some significant differences in inhibiting the growth of Porphyromonasgingivalis bacteria (p<0.005) between each treatment. Conclusion: Edible chitosan film from white shrimp (Litopenaeusvannamei) scalp waste can inhibiting the growth of Porphyromonasgingivalis bacteria.