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Increasing threat of pollution in recent times may have a direct or indirect effect on the microbial flora of fish which in turn may cause a threat to human health due to frequent consumption of the fish. Grass carp (Ctenopharyngodonidella) were collected and analyzed for the presence of different microbial organisms from their gut content. Microbial counts were analyzed in the fish skin, gills and gut. The intestinal gut showed the highest biome of bacteria. Pseudomonas spp., Aeromonas spp., Salmonella spp., and Clostridium botulinum were identified in 55%, 25%, 12% and 8% respectively from the gut biome. We found the occurrence of human pathogenic Pseudomonas sppin the gut of grass carp were confirmed to be in higher count, which may affect the human health on consumption. The result analyzed were found to be statistically significant P<0.05.