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AIM: The aim of the study is to find the effect of Isotonic exercise (walking on treadmill) on Intraocular pressure. To find the association of exercise on intraocular pressure.MATERIALS AND METHODS: It is a prospective study conducted in the department of ophthalmology Meenakshi Medical College and research institute, Enathur, Kanchipuram from June 2019 – December 2019. Medical students satisfying inclusion criteria are selected, procedure were explained, consent for the study were taken.RESULT:In our study we noticed significant reduction in intraocular pressure measured immediately after an isotonic exercise (walking in treadmill for 30 minutes) in all students. CONCLUSION: our study showed a significant reduction in IOP and glaucoma is the second leading cause for blindness. So by this study we stress the importance of including exercise as an adjunct to anti-glaucoma agents for treating glaucoma.