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Colon cancer is considered a malignant neoplasm that ranks third after breast and lung cancer in terms of its prevalence in the world. It is the second cause of death for women, the third for men, and the fourth in the world. Blood samples (100) were collected from the local Baghdad Hospital. they were divided into two groups. The first group includes 30 patients of colon cancer ages ranging from (20 - 40) with 20 healthy people as the control group as the same age and the second group includes 30 patients of colon cancer ages (41 - 60) with 20 healthy as control. Biological evidence to help in the early diagnosis of the disease was measured. The measured level of enzymes (Tyrosine kinase, Ornithine Decarboxylase, 5'-Nucleotidase, Gamma-Glutamyl Transpeptidase) for the two groups with their control also and from the results showed an increase in the concentration of enzymes in the serum of colon cancer patients compare with the control group.