Isolation and Purification of Endophytic Actinomycetes Spp from Local Algaein the Central Marshesof Thi-Qar Provence, Southern of Iraq

Abstract

A total of fifty local green algae samples were collected from three different areas fromcentral marshes of Thi-Qar Provence, southern of Iraq.The current study, which continued to Isolation, purification and characterization endophytic actinomycetesStreptomycets sp. from local algaeisolates (chlorophyta), (Chladophora sp. and Ulothrix sp.).The Endophytic Actinomycetes were isolated by starch casein agar(SCA) and Actnomycetes Isolation Agar (AIA) plate method.Various biochemical testes performed for the identification of potent isolates. Identification of the strainswere carried out by a conventional PCR protocol to analyze simultaneously the presence of 16S rRNAgene. Three endohpytic actinomycetes isolates has ben tested for the antimicrobial activities against multidrug resistant pathogens (bacteria and yeasts) by the cross streak technique.The extracellular extract crud of the isolates (S5) MT974060 (Streptomycets) recorded great zone of inhibition than other isolates (17.0, 11.5 and 10.0 )mmagainst Enterococcus faecalis, Bacillus subtilis and Klebsiella sp. respectively. While (14.0, 12.0 and 18.0)mm against E.colli,Proteus sp and Pseudomonas sp. respectively.From the results, it was suggested that use of these isolates to produce new antibiotics against pathogenic bacteria, fungi and other pathogens.