Protective Effect of Morin Hydrate in Humanepidermal Keratinocyte Cell Line (Hacat) Upon Exposure to Uvbirradiation Induced Oxidative Damage and Biochemicalalteration

Abstract

Ultraviolet-B (UVB) 280–320 nm radiation penetrates epidermis and is completely absorbed in the upper dermis of the skin. It causes a several harmful effects which include basal and squamous cell carcinoma, cataracts, sunburn, melanoma, immunosuppression and photoaging of the skin. The incidence of skin cancers has been increasing worldwide in the last few decades. Thus, identification of more and appropriate potential drug targets is essential for the prevention of skin cancers. Hence, the present study was carried out to investigate the protective effects of morin in human epidermal keratinocyte cell line (HaCaT) upon exposure to UVB irradiation induced oxidative damage. HaCaT cells were pretreated with morin (50 μM) for 30 mins before UVB irradiation. Several cellular and oxidative end points parameters were analyzed. UVB irradiation pretreated with morin (50 μM) showed significant increase in the levels of antioxidants and lipid peroxidation, and DNA damage and apoptotic morphological changes were manifest drastically in HaCaT cells. The present study was demonstrating protective effect of morin against UVB radiation.