The Effects of 900 MHz Electromagnetic Radiation CD4+ T cells, IL-2, IL-10, and IL-17a in Peripheral Blood Mononuclear Cells cultures
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Abstract
The immune system is able to react to discrete environmental stimuli like electromagnetic field (EMF). To investigate the effects of radiofrequency EMF (RF-EMF) on T helper cells, our study aimed to determine that exposure to 900 MHz RF-EMF affects Th1, Th2, and Th17a cells in peripheral blood mononuclear cells (PBMC) cultures. The PBMC cultures from healthy subjects were exposed to 900 MHz RF-EMF (38 V/m, SAR 1.194 W/kg) with durations of 15, 30, 45, and 60 minutes and distances of 0 and 5 cm or without irradiation, respectively. After 48 hours culture, the effects of RF-EMF exposure on the number of CD4+ T cells, IL-2, IL-10, and IL-17a were evaluated using flow cytometer. There was a decreased CD4+ T cells at 15 & 30 minutes exposure and, which then became almost the same at 60 minutes exposure, to those without. IL-2 and IL-10 increased significantly, but decreased with the duration of exposure, approximates the control. Interleukin-17a was decreased significantly compared to controls. The effect of 900 MHz RF-EMF exposure on the percentage of CD4+ T cells, IL-2, and IL-10 was temporary. The RF-EMF causes reduction of IL-17a.