Screening, Production and Characterization of Bacterial Strain for Industrial enzymesa their Potential application in Biodegradation of Plastic and Paper Waste

Abstract

Enzymes are widely used in industries like Food, Paper, Textile etc. Recent years focused on recycling and utilising waste efficiently. Numbers of studies based on utilizing agricultural waste, municipal and related waste have been reported. Present study focuses on utilization of vegetable market waste as substrate in order to produce industrially important enzymes. For this, the sample, vegetable market waste was collected from vegetable waste dumping site, Chromepet, Chennai, Tamil Nadu. Bacteria were isolated from it andpure cultures obtained were tested for the production of five different industrially important enzymes namely: protease, amylase, cellulase, lipaseand pectinase. One bacterial strain which was later identified as Bacillus aryabhattai by sequencing 16srDNA regions, that produced most of these enzymes has been selected and used for comparative analysis of enzyme activity of the above mentioned five enzymes produced in both specific media and vegetable market waste media. Results have shown that protease and cellulase produced in vegetable market waste media has 83% and 72.3% higher enzyme activity respectively than their activity in specific media. Enzyme activity of lipase is 50% less in vegetable market waste media whereas enzyme activity of amylase is similar in both the media.