Phenotypic and Molecular Detection of Streptococcus Uberis Isolated From Milk of Subclinical and Clinical Mastitis of Cow in Basrah City

Abstract

Streptococcus uberis is found as commensals on the mucosa of the tonsils and intestinal tract of cows and responsible for approximately 20-30% of clinical mastitis cases in dairy cows .S.uberis is a gram positive coccus facultatively anaerobic and catalase negative and the key characteristics of S.uberis are the hydrolysis of esculine and production of acid on inuline ,S.uberis is responsible for infection in clinical and subclinical mastitis cows.Atotal of 100 milk samples was collected from clinical and subclinical mastitis cows from different dairy farm in Basrah province for detection of some phenotypic and genotypic characterization and detection of some virulence genes of S.uberis by PCR techniques and biofilm formation were assayed by quantitative microtiter plate. Antibiotic susceptibility performed using conventional assay method. Result revealed that ,The percentage of S.uberis isolated from the total examined milk sample was 46% in studding the phenotypic characteristic on sheep blood agar was found that alpha –hemolysis and negative camp factor like reaction and slime production as indictor for biofilm formation were detected in all the isolates .the study of virulence gene (sua 93.7%)(cfu 66.6%)(hasA 62.5%) in the present study usednine antimicrobial ,amoxillin(58.6%),ampicillin(100%),ciprofloxacin(69.5),ciftrixone(93.4),erythromycin(76%),trimethoprim(95%), gentamycin(100%),tetracycline(10.8),penicillin(45.6%).