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The pyocyanin stain was extracted from Pseudomonas aeruginosa K3 isolated from several food sources, and diagnosed using the HPLC device, and the biological efficacy of the produced pigment was tested on a number of pathological microorganisms. The results of the study showed that there is sensitivity of pathological bacteria against the pseudomonas aeruginosa stain, andits refer to indicated an inhibitory effect for T2 treatment for all types of test bacteria. The T2 treatment (the sample to which the raw pyocyanin e extract was added by 2%) had the highest inactivation by 28, 25 and 20 mm for Escherichia coli, Staphylococcus aureus and Bacillus cereus, respectivelyand the least inhibitory was the direction of Enterococcus faecalisandSalmonella typhimurium reaching 9 and 5 mm respectively. While the comparison treatment T1 (using chloramphenicol solution) showed an inhibitory effect against the test bacteria, and it was 30 mm against Escherichia coli, Staphylococcus aureus and Bacillus cereusrespectively and by 26 mm was the direction of Enterococcus faecalisandSalmonella typhimurium, respectively.