Expression of Transforming Growth Factor – β In Type II Diabetics With Chronic Periodontitis Following Minimally Invasive Periodontal Surgery Under Loupe magnification

Abstract

AIM: To compare and evaluate the expression of Transforming Growth Factor - β (TGF- β) in the Gingival Crevicular Fluid (GCF)following minimally invasive periodontal flap surgery under macro and microsurgical approach during early wound healing in Type II Diabetics with generalised chronic periodontitis (GCP).METHODS: Thirty diabetics with GCP underwent simplified papilla preservation flap surgery under conventional (Control 30 quadrants) and microsurgical approach (Test 30 quadrants). Early Wound Healing Index (EWHI) was evaluated at first and second week post- intervention. GCF was collected at the time of surgery, 2^nd and 6^th week post -surgery for estimation of TGF- β. RESULTS: TGF- β at baseline was 683.78±33.14 ng/L (control) and 694.33±30.06 ng/L (Test). Post-surgery at second week, it increased in both the groups, 1048.47± 133.22 ng/L(test) and 872.51± 155.58 ng/L(control). At sixth week, the sites treated under loupes showed an increased expression (698.50± 48.35 ng/L) with statistical significance when compared to control sites (P<0.001). 100% of the test sites achieved complete flap closure at 2^nd week, whereas it was 9.7% with incomplete flap closure in control sites.  CONCLUSION: Minimally invasive periodontal flap under magnification loupes demonstrated an increased expression of TGF-β and better EWHI scores in diabetics with chronic periodontitis.