An Invitro Cytotoxic Analysis of Citrus Sinensis ( Sweet Orange) as Root Canal Irrigant on Fibroblast Cells

Abstract

Background: Resistant microbes always represent a challenge in the treatments of various well-known infections and urges the need for substances with potent antimicrobial properties. Citrus sinensis is known for its medicinal value. Many medicinal properties of orange peel extract, such as against colic, upset stomach, cancer, diuretic, immuno enhancing, and fight viral and bacterial infections. This study aims to evaluate the cytotoxicity of citrus sinensis against fibroblasts.

Methodology : Aqueous and ethanol extracts prepared from peel of Citrus sinensis were screened for in vitro cytotoxic activity against L9292 cells using MTT cell viability assay. For cell viability assay, the microplates filled with 100 μl of L929 Cells and growth medium using micropipette, read on an enzyme-linked immunosorbent assay (ELISA) reader at 570 nm. Each experiment was carried out in triplicate and the IC50 of the test samples as the percentage survival of the cells was calculated.

Results: CSE did not adversely affect the fibroblasts even up to 50% concentration showing a nontoxic effect even till 200 μg/ml dose in comparison with CHX on these cells.

Conclusion: Citrus sinensis peels extract demonstrated less cytotoxic activity warranting further in vivo clinical studies to determine the exact dosages as root canal  irrigant and its effectiveness in practical situations.