Toxicopathological Experimental Assessments of Median Lethal Dose (LD50) of Dimethylformamide on Male Reproductive System of Albino Rats (Epididymis)

Abstract

This experiment aimed to investigate the Toxicopathological effect of N, N-Dimethylformamide (DMF) (LD50) on the adult male rats reproductive system (epididymis) in acute and sub-acute exposure in male rats. Twelve male rats used in the study, six rats used to estimate the acute toxicity (LD50), While the other group Normal diet and water for twenty-four hours. The experiment has lasted for twenty-four hours at the end of the experiment all animals killed, epididymis was kept in 10% formalin used for histological examinations. results revealed thinking of the capsule of the epididymis absent of sperm from the lumen with mild infiltration of inflammatory cells, other sections showed decreased in the high of the epithelia covered the epididymis tubules with the absence of microvillus with stasis of abnormally shaped sperm inside the lumen. conclusion: there was the histopathological effect of  DMF on the male reproductive system of rats (epididymis) with its different LD50 dose.