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This study was aims to assess the effect of laser diode light at 532nm on susceptibility ofKlebsiella pneumoniae isolates from burn and wound infection to antibiotics. The susceptibility of three isolates of K.pneumoniae (T40, T92 andT105) provided from previous study were tested against nine antibiotics. All three isolates were showed sensitivity to Imipenem (IMP) and Ciprofloxacin (CIP) while it’s showed resistance to the rest antibiotics. The effect of the radiation on the susceptibility to antibiotics were tested via subjecting isolates laser diode light at 532nm wavelength 3mW, for 1, 2 and 3min.Results showed that a significant increase in susceptibility for all isolates toward imipenem and ciprofloxacin.