Using of Uv Light Type C and Growth Regulators in Propagation and Cormels Formation in Gladiolus Spp

Abstract

Micropropagation of Gladiolus spp. (Primulinus) from axillary buds of flowers stalk as a source of explants. Axillary   buds were sterilized under aseptic conditions and cultured in (MS) media supplemented with various levels of growth regulators (BA, IBA). The plantings were taken and exposed to UV  light type C radiation with length 254 nanometer at different periods of time (30, 60, 90 second) . The effect of BA on the vegetative multiplication of buds was studied with concentrations (0.0, 0.25, 0.50 and 0.75) mg / liter, the best concentration for multibecation was 0.5 mg l.  IBA with concentrations (0.0, 0.25, 0.50 and 1) mg / liter were used for roting and cormels formation, the best result present at the concentrations 0.50 mg/L. The result showed that UV light at time of exposure 60 sec   gave the highest response to multibecation and cormels formation.