Phytochemical Screening by FTIR Spectroscopic Analysis and Anti-Bacterial Activity of Methanolic Extract of Selected Medicinal Plant of Anethum Graveolens and Plantago Major
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Abstract
The objectives of this study were analysis of the secondary metabolite products of of Anethum graveolens and Plantago major and evaluation of anti-bacterial activity. The FTIR analysis of Anethum graveolens proved the presence of functional assignment Alkenes, Alkyl halides, Amide, and Alkane with Peak (Wave number cm-ˡ) 667.3 Bending (Strong =C–H Bond), 758.0 Bending (Strong =C–H Bond), 1026.1 Stretch (Strong C-F Bond), 1238.6 Stretch (Strong C-F Bond), 1317.2 Stretch (Strong C-F Bond), 1375.2 Stretch (Strong C-F Bond), 1608.5 Stretch (Strong N-H Bond), 1732.1 Stretch (Strong C=O Bond), 2850.4 Stretch (Strong C-H Bond), 2920.9 Stretch (Strong C–H Bond), The FTIR analysis of Plantago major proved the presence of functional assignment Alkenes, Alkyl halides, Amide, and Alkane with Peak (Wave number cm-ˡ) 1001.06 Stretch (Strong C-F Bond), 1049.28 Stretch (Strong C-F Bond), 1238.30 Stretch (Strong C-F Bond), 1386.82 Stretch (Strong C-F Bond), 1394.53 Stretch (Strong C-F Bond), 1517.98 Stretch (Medium C=C Bond), 1635.64 Stretch (Bending N-H Bond), 2854.65 Stretch (Strong C-H Bond), 2926.01 Stretch (Strong C-H Bond). Zone of inhibition (mm) of test bacterial strains to Anethum graveolens bioactive compounds and standard antibiotics were (5.001±0.31), (4.006±0.23), (4.961±0.21), and (3.996±0.20) uses Anethum graveolens bioactive compounds, and (2.103±0.11), (2.431±0.11), (2.011±0.11), and (1.027±0.01) uses Rifambin, and (1.732±0.10), (2.009±0.11), (1.417±0.10), and (1.915±0.11) uses Kanamycin for Staphylococcus aureus, Escherichia coli, Proteus mirabilis, Klebsiella pneumonia respectively. Zone of inhibition (mm) of test bacterial strains to Plantago major bioactive compounds and standard antibiotics were (4.591±0.21), (5.014±0.30), (4.992±0.30), and (4.087±0.21) uses Plantago major bioactive compounds, and (3.811±0.21), (3.001±0.19), (1.961±0.12), and (1.063±0.10) uses Rifambin, and (2.007±0.10), (2.038±0.11), (2.001±0.10), and (2.075±0.11) uses Kanamycin for Staphylococcus aureus, Escherichia coli, Proteus mirabilis, Klebsiella pneumonia respectively.