Investigation of Toxic Secondary Metabolites Produced by Some Dermatophytes

Abstract

The study included 71 samples of hair fragments, nail clippings, and skin scrapings  which were collected from patients whom admitted to Department of Dermatology at Al-Diwaniyah Teaching Hospital during the period from March 2018 to August 2018, all specimens were examined by direct KOH 10% mount smear and cultured in sabourauds dextrose agar to determine the dermatophyte species.The laboratory examination results showed that out of 71 samples processed,49 (69%) were positive in both KOH mount and culture . The main causative agents of human dermatophytes were as follow:Trichophytonrubrum 31(63.3%), Trichophytonmentagrophytes 11( 22.4%), Microsporumcanis 5(10.2%) and Epidermophytonfloccosum 2(4.1%) . Trichophytonrubrumwas selected for its ability of toxic secondary metabolites production based on the efficiency of production among tested dermatophytes.Thin layer chromatography results of chloroform extracts revealed three spots with different colors named as follows; TRA compound (dark blue), TRB compound ( dark brown), and TRC compound (light blue) under UV light have different with rate of flow values equal to 12.1, 48.5, and 84.8 respectively .