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Cassia auriculata is used extensively in the indigenous system of medicine as an anti-diabetic agent. An eco-friendly green synthesis of iron oxide nanoparticles using leaf extract of Cassia auriculata leaf was investigated. UV visible spectra showed the maximum absorbance of 285 and 324 nm due to the excitation of surface plasmon vibrations in the iron oxide nanoparticles formation. FTIR spectrum exhibited the characteristic band at 618 cm-1 which indicated the Fe-O stretching of Fe2O3 nanoparticles. The sharp peaks indicated the crystallinity and purity of iron oxide nanoparticles. The average particle size of the synthesized iron oxide nanoparticles was estimated to be 47 nm using the Scherrer equation. Transmission electron microscope (TEM) image of iron oxide nanoparticles showed that the nanoparticles size was below 20 nm.