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Sports, despite its many benefits to athletes and the general public, can also cause injuries. One of the most common of these injuries is delayed onset muscle soreness which often occurs as a result of extroverted activities in all individuals, especially during strenuous exercise. Therefore, this study aimed to investigate the effect of short-term use of portulaca oleracea supplement on the index on delayed muscle contusion in women. The present study is quasi-experimental in terms of method and applied in terms of effect. For this purpose, 20 active female subjects (who had three training sessions per week) were divided into an experimental group (n = 10) and a placebo group (n = 10). Subjects took 1200 mg of portulaca oleracea supplement daily for 7 days from 72 hours before to 72 hours after exercise. The research hypothesis was analyzed using the statistical method of analysis of variance with repeated measures (factor design within and between groups). After observing a significant difference, the Ben Foroni post hoc test was used. The significance level was also determined for all calculations (p <0.05). The results of the research showed that; The concentration of lactate dehydrogenase, which is a major biochemical marker of muscle contusion, was significantly reduced in the experimental group (portulaca oleracea supplement) compared to the placebo group. Hence it was observed that; Portulaca oleracea supplement reduces muscle soreness.