Β-Sitosterol Augment Antihyperlipidemic Effect in Hyperlipidemic Rats Induced by triton

Abstract

AIM:β-sitosterol augment antihyperlipidemic effect in hyperlipidemic rats induced by Triton. MATERIAL & METHODS:Leaves of Corchorus trilocularis were extracted by using chloroform and other solvents. Since Chloroform extract showed better activity as compared to other extract so it was selected for further isolation and characterization of active compound. The compound was isolated by using column chromatography and suitable solvent systems i.e. chloroform: methanol (74:26). β-sitosterol was isolated by using this solvent system. Its acute toxicity studies were performed for the dose determination and further antihyperlipidemic evaluation.RESULTS:IR (KBr) 3460, 2868, 1731, 1644, 1512, 1455, 1421, 1350, 1298, 1249, 1201, 1108, 950, 849, 726, 552cm^{-1 1}H NMR (400 MHz, Chloroform) δ 5.48 (s, 5H), 3.42 (s, 5H), 2.25 (s, 4H), 2.02 (s, 4H), 1.88 (d, J = 18.0 Hz, 9H), 1.77 (s, 4H), 1.70 (d, J = 9.2 Hz, 11H), 1.66 – 1.56 (m, 15H), 1.48 (dd, J = 15.0, 7.2 Hz, 21H), 1.42 – 1.12 (m, 72H), 1.16 – 1.12 (m, 18H), 1.15 – 1.08 (m, 27H), 1.08 – 0.90 (m, 92H), 0.85 (s, 6H), 0.73 (s, 4H). ¹³C NMR (100 MHz, Common NMR Solvents) δ 141.20 (s), 121.64 (s), 71.05 (s), 57.01 (s), 56.46 (s), 51.13 (s), 45.02 (s), 43.08 (d, J = 9.0 Hz), 39.72 (s), 37.77 (s), 37.16 (s), 36.72 (s), 35.41 (s), 32.66 (s), 32.31 (s), 31.60 (s), 31.13 (s), 28.32 (d, J = 4.1 Hz), 24.97 (d, J = 2.6 Hz), 21.27 (s), 19.99 – 19.67 (m), 18.84 (d, J = 13.2 Hz), 13.00 (s), 12.11 (s). β-sitosterol at the dose of 10 and 20 mg/kg showed highly significant (p<0.001) and moderately significant (p<0.01) effect on lipid profile in comparison to that of hyperlipidemic group. CONCLUSION:The noticed antihyperlipidemic impact might be because of improved glycemic control and expanded plasma insulin movement, which permits the rodents treated with β-sitosterol to keep up plasma and tissue lipids in ordinary level.