Immune Status of Osteoarthritis Patients

Abstract

This study was performed for investigating of immune status among osteoartgritis patients through serological measurement of interleukin-1 (IL-1), IL-10, monocyte interferon gamma inducing factor (MIGF), p53 upregulated modulator of apoptosis (PUMA), transforming growth factor-beta (TGF-β), tumor necrosis factor-alpha (TNF-a) using the sandwich enzyme-linked immunosorbent assay (ELISA). Totally, 160 people involved 120 osteoarthritis patients and 40 healthy ones as a control, were subjected to sampling of venous blood. The total findings showed a significant elevation in values of IL-1 (147.5 ± 4.689 pg/ml), MIGF (241.5 ± 15 pg/ml), PUMA (57.70 ± 0.5358 pg/ml), and TNF-a (42.91 ± 0.839 pg/ml); whereas, there were significant decreases (p<0.0338) in values of IL-10 (102.6 ± 3 pg/ml) and TGF-β (91.56 ± 0.7646 pg/ml). Regarding age factor, patients of ³36 years were reported a significant increase (p<0.0086) in values of PUMA (53.09 ± 0.8555 pg/ml) and TNF-a (44.89 ± 1.309 pg/ml), and a significant decrease (p<0.0179) in values of TGF-β (86.51 ± 0.9277 pg/ml) when compared to those of £35 years (49.72 ± 0.8222 pg/ml, 40.93 ± 0.9679 pg/ml, and 96.61 ± 0.4472 pg/ml, respectively). However, no significant variation (p<0.4556) was observed between both age groups (£35 years and ³36 years) relation to IL-1 (149.2 ± 6.188 pg/ml and 145.8 ± 7.116 pg/ml, respectively), IL-10 (102.9 ± 4.106 pg/ml and 102.3 ± 4.426 pg/ml, respectively) and MIGF (262.1 ± 18.31 pg/ml and 222.4 ± 22.26 pg/ml, respectively). Concerning gender, higher values of IL-10 was reported in females (112.9 ± 3.422 pg/ml) than males (92.25 ± 4.389 pg/ml), (p<0.0002); while, values of MIGF and PUMA were increased in males (270.2 ± 18.46 pg/ml, and 54.68 ± 0.7729 pg/ml, respectively) when compared to females (214.3 ± 21.09 pg/ml, and 48.13 ± 0.6383 pg/ml, respectively), (p<0.0271). However, insignificant variation (p<0.9296) in values of IL-1, TGF-β and TNF-a was appeared between males (143.9 ± 5.975 pg/ml, 91.70 ± 1.092 pg/ml, and 42.05 ± 1.178 pg/ml, respectively) and females (151.1 ± 7.260 pg/ml, 91.42 ± 1.084 pg/ml, and 43.78 ± 1.194 pg/ml, respectively). In conclusion, significant positive correlation between immune markers and osteoarthritis was confirmed in this study. However, furthermore studies are of great importance to support effect of other immune markers in initiation, degradation as well as healing of osteoarthritis.