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The current study was performed at Malakandher farm, The University of Agriculture Peshawar during the year 2021 to assess the population density of L.erysimi and their related natural enemies in adding to the comparative resistance determination against L.erysimi in different Brassica lines and their responses to its related natural enemies. Three different Brassica lines Hashmi 13, Duree e NIFA Peshawar and Pre basic Peshawar were studied, which were triplicate in Randomized Complete Block Design. The maximum population of aphids per leaf was observed during 3rd week of February and minimum population of aphids per leaf was observed during 5th week of February at all brassica lines. Amongst all the Brassica lines, Pre basic Peshawar reportedrelatively more resistance in comparison to Duree e NIFA Peshawar and Hashmi 13. Pre basic Peshawar recordedminimum resistance towardsL. erysimi, which was reported to be the most susceptible line of canola. Pre basic Peshawar was also noticed superior in case of natural enemies. Peakpopulation of all natural enemies was observed on this variety followed by Duree e NIFA Peshawar. Leastpopulation of natural enemies was reported by Hashmi 13.