Intraspecific Genetic Diversity in Different Capsicum Frutescense Germplasm Revealed by Molecular Markers

Abstract

The Capsicum frutescence is one of the major spice crop that belongs to family Solanaceae. Capsicum is a highly value crop, as a fruit it is rich in vitamin B, C & E. The objective of this study was to show genetic diversity within different Capsicum frutescence germplasm by using DNA markers. ISSR and RAPD molecular marker used for the analysis of the genetic diversity of Capsicum frutescence. These markers involve Polymerase Chain Reaction (PCR) amplification of DNA using a single primer composed of a microsatellite sequence. ISSR and RAPD technology rapidly reveals high polymorphism and determines genetic diversity. Among 15-20 primers used only ISSR primer UBC 815 and RAPD primer  B-20 amplified alleles showed the specific polymorphism within the germplasm of chilli.