Detection of Malaria through Light Microscope with Fluorescent Microscopy and Interference Filter

Abstract

The Aim of the Current study to compare Giemsa staining with acridine orange (A.O) staining by using light microscopy with IF and also with fluorescent microscopy for diagnosis of parasites in peripheral blood of patients suffering from clinically suspected cases of malaria at Saidu Teaching Hospital Swat Pakistan. in this study 350 patients with fever and shivering were included. General investigations like Hb, TLC and platelets were done by sysmex K-1000. Thin and thick blood films were made and stained according to protocol given i.e. by   Giemsa and AO stains and slides were examined by different microscopes i.e. light microscope, light microscope with IFS and fluorescent microscope. Out of 350 subjects, 297 (85%) patients showed positive parasitemia and 53(15%) subjects were negative for malaria parasites. fib, TLC and platelets were reduced when comparing with MP negative cases. IFS microscope with acridine orange staining showed early detection of malaria parasites by counting fewer fields as compared to light microscopy with Giemsa stains. Time consumed for detection of parasites was also significantly   reduced in IFS microscope by using AO stains.