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Wheat is predominantly used as a staple food all over the world. Stem rust resistance by Sr22 gene is researched and confirmed before but in the current study, its molecular function is predicted by using different in-silico tools such as ExPASy-Translate, ExPASy- PortParam, and InterProScan. We investigated the in-silico functional compositions of Sr22 protein, and the results revealed that it belongs to the Plant Disease Resistant family and is comprised of Leucine-rich repeats at C-terminal and Adenosine 5'-Diphosphate Binding domain at N terminal. As an ADP binding protein, it can arrest fungal growth by inhibiting the function of nucleic acid. Moreover, Sr22 protein is also known to involve in regulating programmed cell death (PCD).