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3- ( 4,5- dimethylthiazol - 2- yl ) - 2,5- diphenyltetrazolium bromide (MTT) method is used to study the cytotoxicity effect of Partial Purified Proteins Saccharomyces cerevisiae and lactic Acid Bacteria on HT-29 Cell Line. The results showed all concentrations (6.25, 12.5, 25, 50,100 and 200µg/ml) of partial purified protein caused a significant inhibition rate of HT-29 after 72 hrs. Exposure and reached to 2.333, 9.667, 20.33, 37.00, 51.33 and 77.33 % respectively with statistically significant differences at P≤ 0.05 in comparing to control. While the result of cells viability (97.7, 90.3, 79.7, 63.0, 48.7and 22.7) % respectively when exposure (6.25, 12.5, 25, 50, 100 and 200 µg/ml) of Partial Purified Protein were used. Best fit value in HT-29 cell line is (37.39) µg/ml which represents a IC50 which is the lowest concentration that kills 50% of cells and the cells after treatment, noticed that they shrink, and their number and size reduced. From these results can concluded that the synergistic partial purified proteins of S.cervesiae and lactic acid bacteria have the effective role in treating cancer through its inhibitory effect And lethal toxicity on colon cancer cells line.