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Toxic metabolites of fungi or mycotoxins have been responsible for many epidemics in human and animal communities, especially in recent times. Samples were collected from cultivation and procreation centers in northern Iran and then isolated and recognized on five substrates CYA, YES, MEA, SYES and OA. The subspecies of Wentii, Flavi, Nigri, Circumdati,Candidi, and a number of unknown species were cultured on CGM / CM / DRYE triplicate media for motivation of toxicity and measurer. Therefore provisioned of theextract growth media and measured a amount of aflatoxin arrived to culture medium with CZ MEZ Eliza method. Most of the samples were from plantations and factories in eastern Guilan. In species segmentation, the highest number of species after unknown species was related to flavus species. There was a significant difference between the level of toxin was proportional to sector and species and also between aflatoxin levels measured at biomass and the culture medium (P <0.05).Due to the fact that most of the samples were obtained from fields and factories in East Gilan, these areas are high risk in terms of aflatoxin isolate contamination.