Immunological Study for the Changes that Associated with Infection of Mice with Shiga Toxin Producing Escherichia coli (STEC) O157:H7

Abstract

Present study discussed the immunological effect of STEC E. coli O157:H7 on mice. Sixty Albino mice, both sexes with age between(6–8) weeks  of old were divided into two  groups: ''infected group'' (30) were inoculated with infectious dose orally , and ''control group'' (30) were given phosphate buffer saline orally.

The immunological feature,  mice of the infected group revealed both  of the humoral and cellular immune response. The HI response was detected by  the passive haemagglutination test and the results revealed the higher  titer of antibodies is about (253.0±21.70) at 28 day after  infection. The CI response was detected by the skin test, the mean thickning of the right footpad was (3.03±0.15 mm) after about one day then dropped to reach (1.80 ±0.09 mm) after 3 days in mice with infectious dose.

The cytokines level was measured by Sandwich ELISA (Interleukin 10 (IL10) and interferon gamma (IFN- γ) ) which showed a major increase (P<0.05) in the  comparison with control group at all experiment period (14, 21 and 28) days post infection.

In conclusion, The investigational infection with STEC O157:H7 activated both CI and HI response with raised of inflammation cytokines (IFN- gamma and IL-10).