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Lipid abnormality is one of important risk factor for cardiovascular disease in AMI patients. In particular, LDL-C and HDL-C is an important factor for atherosclerosis and cardiovascular disease development. Lipid profile is not that much experienced with cardiovascular diseases (Acute myocardial infarction) in Pakistan.The purpose of the study was to investigate the serum lipid levels in both elderly and non-elderly AMI patients for both male and female. A total of 265 patients with myocardial infarction were collected in Jan 2019 from different Hospitals, Lahore, Pakistan. Demographic Information of each participant that’s age, sex, were recorded as well. Lipid profile parameters were done as; Selectra Pro-M and AMP Diagnostic kits were used according to the manufacturer guidelines. After drawing a sample of 5ml from each patient into clot activator tube centrifuged the sample at 8000 rpm for 10–15 min, serum sample was putted in a selectra serum cup and then placed that cup in automated selectra rooter and later been performed according to the standard operating protocol of the selectramachine.In this study there were 265 patients with AMI. The mean age was comparable between non-elderly and elderly ; 52 years for men in non-elderly and 72 years for men in elderly, 54 years for women in non-elderly and 75 years for women in elderly). Levels of TC, LDL, and TG were lower in elderly than in non-elderly for the males and found significant (<0.05) except HDL whom level was comparable. Female lipid profiles between non-elderly and elderly, which containing TC, LDL, HDL, and TG, were also found in-significantly (> 0.05). However, in the non-elderly group, there were no significant differences in TC, LDL, and TG between male and female except HDL, whereas, in elderly group all the lipid parameters were found drastically significant. In addition, as we discussed female were drastically increased than male in this study for most of the lipid parameters, we also compared male elderly and female non-elderly which were noted as insignificant as well. Similarly, as for as female elderly is concern against the male non-elderly individuals the Anova compare mean test depicted a comparable association in between (>0.05).Dyslipidemic feature is pre-dominantly frequent in the non-elderly than elderly individuals for males. Serum Levels of all lipid parameters including TC, LDL, HDL, and TG were found raised in females than males for the elderly Pakistani individuals. Consequently, these patients may require lipid-lowering therapy to overcome the increased risk of cardiovascular disease. Another thing is that high TG which should be reduced as we found overall TG as independent factor for AMI in this study, so it may be critical to such patients.