Antibiotic Susceptibility Pattern of Clinical Isolates of Methicillin Resistant Staphylococcus Aureus in Peshawar, Pakistan

Abstract

The Staphylococcus aureus are facultative anaerobic gram-positive cocci that arrange in grape like bunch of irregular clusters. On the base of enzyme coagulase, the organismhaspotential to clot blood plasma; staphylococci are separated into coagulase positive staphylococci and coagulase negative staphylococci. Coagulase-positive staphylococci include pathogenic organism named S. aureus. MRSA is recognized as a major cause of nosocomial infections ranging from minor skin infection to life threatening disease. The emergence of MRSA strains is an alarming situation for both hospitalized and non-hospitalized patients.The current study was aimedto find the prevalence and antibiotic susceptibility pattern of MSSA and MRSA from clinical isolates.  A total of 100 clinical isolates of Staphylococcus aureus was collected from indoor patients of Khyber Teaching Hospital Peshawar. The isolation and identification of these clinical isolates were done using standard microbiological methods as per CLSI guidelines. The Antibiotic susceptibility pattern was done by using disk diffusion method. Out of 100 clinical isolates, 18(18%) were MRSA and 82(82%) were MSSA. Prevalence of MRSA was same in male and female. MSSA showed higher susceptibility to doxycycline (98.0%) while AMC (29.0%) showed lowest susceptibility against S. aureus. MRSA was higher sensitive to Vancomycin and Amikacin (94.4%) but resistant to cefoxitin (100%). Thecurrent study concludes that 18% isolates were MRSA in indoor patients of MTI KTH while the remaining isolates were MSSA.