Eco Friendly Synthesis, Characterization and Optimization of Silver Nanoparticles using Polyherbal Formulation and its Invitro Analysis for Antidiabetic Study in L6 Cell Lines

Abstract

Green synthesis is the method of synthesising nanoparticles mediated through plant sources. For the synthesis of silver nanoparticles (AgNPs), an economically viable and “green" approach has been devised, which has been studied using UV-Visible absorption spectroscopy, FTIR, XRD, and SEM, as well as in vitro anti-diabetic efficacy to analyse the hypoglycaemic plants. The plants used for the present study includes a formulation of Mangifera indica, Vinca rosea and Allium sativum. These chosen plants showed higher a profile of efficient hypoglycaemic nature in many other studies. This polyherbal formulation is prepared with ethanolic extracts and further mixed with the silver nanoparticles. The colour shift and prominent peak in UV-spec analysis indicate that silver nanoparticles have been synthesised effectively. The FTIR research reveals the phytochemicals responsible for the reduction of silver and iron nanoparticles. The presence of silver and iron nanoparticles was crystalline, according to XRD measurements while the surface morphology and size were determined via SEM examination. The alpha-amylase inhibition assay and the percentage of inhibition with standard ascorbic acid were used to determine anti-diabetic efficacy using L6 cell lines. As a result, the study of green silver nanoparticle synthesis and its applications can be expanded to include a wide range of plant species to treat different ailments eliminating harmful side effects.