“Gadose” Isolation And Structure Elucidation Of Novel Octasaccharide From Gaddi Sheep’s Milk By

Abstract

Milk Is An Essential White Fluid In Glycobiology Which Contains Number Of Important Nutrients Like: Carbohydrate, Protein, Fats Etc. Carbohydrate Present In Milk Is Either In Free Form Or As Glyco-Conjugates. Milk Is One Of The Rich Sources Of Biologically Active Oligosaccharides In New Born Which Protect From Various Disease. Milk Is Full Of Various Biological Activities Such As Antitumor, Anti-Inflammatory, Anti-Cancer, Antimicrobial Activities. To Discovered More Novel Biologically Active Milk Oligosaccharides, Gaddi Sheep Milk Was Collected And Processed By Modern Kobata And Ginsburg Method Proceed By Gel Filtration And Column Chromatography Which Resulted In The Isolation Of Novel Milk Oligosaccharide Namely Gadose. The Structure Of Isolated Novel Oligosaccharide 'Gadose" As An Octasaccharide Was Elucidated With The Help Of Hplc, Nmr (1h, 13c, 2d Nmr: Cosy, Tocsy And Hsqc) Techniques And Mass Spectrometry And Further Confirmed By Chemical Degradation. The Structure Of Gadose Is-