Resveratrol ameliorating the liver functions against hydrogen peroxide exposed rats

Abstract

This study is designed to investigate the effect of resveratrol supplement on liver injury induced
by hydrogen peroxide in adult rats over 45 days. Forty adult male rats were divided into four equal
group as follows: Controls were given distilled water, group G1: Received tap water containing
0.5% hydrogen peroxide, group G2: Received tap water containing 0.5% hydrogen peroxide and
gavaged resveratrol 87mg/kg B.W. orally and group G3 gavaged resveratrol 87mg/kg B.W. orally.
Blood samples were taken at pretreatment, after 45 days. Serum alanine aminotransferase (ALT),
aspartate aminotransferase (AST), alkaline phosphatase (ALP) and lactate dehydrogenase activities
, as well as total serum protein (TSP ), albumin and globulin concentrations were measured. At day
45 rats were euthanized and liver samples were taken to estimate malondialdehyde (MDA) and
superoxide dismutase (SOD) concentrations. The results showed a significant increase in ALT,
AST, ALP and LDH activities, while a significant decrease in TSP, albumin, globulin
concentrations accompanied with a significant increase in MDA and decrease in SOD in liver
tissues in rats treated with 0.5% hydrogen peroxide. Whereas, resveratrol in group G2 caused an
alleviation of the studied criterions related to hepatic dysfunctions through lowering the
accumulative negative effects of hydrogen peroxide. Our results clarified that resveratrol, by
multiple antioxidant mechanisms interplay, ameliorate liver injury induced by hydrogen peroxide
in rats.