Isolation and Purification of the Nerve Growth Factor from the Murine Submaxillary Salivary Glands Simple method for isolation the NGF peptide

Abstract

The findings from analysis of various classes of the vertebrata and a human being demonstrated the highest
accumulation of the nerve growth factor (NGF) in the submaxillary salivary glands of the adult male mice. The study
aimed at isolation and purification of the NGF from submaxillary salivary glands of male mice by means of the
simplified method for its biological activity to be tested.
Materials and methods. The adsorption chromatography was used to purify the fractions extracted from the
submaxillary salivary glands of mice. Separation of proteins based on their electrophoretic mobility was performed
using Laemmli SDS PAGE procedure. Biotesting of the fractions was performed on the culture of chick embryo spinal
ganglia.
Results and discussion. Three fractions were obtained by means of the adsorption chromatography on glass.
The electrophoretic separation in the polyacrylamide gel demonstrated the presence of a protein with molecular mass of
13 kDa in the acetic acid fraction. Further testing demonstrated the neurites’ growth of spinal ganglia from the chick
embryos scored +4 by the Fenton’s 5-point scale.
Conclusion. Thus, the stable peptide with nerve growth activity from the murine submaxillary salivary glands
was obtained in a single chromatographic cycle.