Juvenile Dysmenorrhea as a Genetically Determined Condition in the Presence of Criteria of Undifferentiated Connective Tissue Dysplasia in Uzbek Women

Abstract

The aim of the study was to identify the COL1A1 and G2046T genes in the presence of criteria for undifferentiated connective tissue dysplasia in Uzbek women with juvenile dysmenorrhea. A clinical examination of 230 girls, with signs of CTD -136 and without it-56, the control group consisted of 50 healthy girls with normal menstruation, and a genetic examination of 118 girls aged 13 to 18 years, with signs of CTD-64 and without it-54, the control group consisted of 68 healthy girls with normal menstruation. Determination of free and bound oxyproline in urine was carried out according to the P.N. Sharaev’s method. It is revealed that with the aggravation of the severity of dysmenorrhea, the criteria for CTD are increasingly manifested. The study of the association of the COL1A1 G2046T genotype revealed a significant increase in the mutant TT genotype in the group of girls with JD with CTD, compared with practically healthy individuals. Analysis of the distribution of allelic variants of the COL1A1 G2046T gene showed that in the group of patients with DM accompanied by CTD significantly higher than in the control group of practically healthy individuals, there was only a tendency to the reliability of alleles, but they did not reach true significance. Correlation analysis between the excretion of oxyproline and the degree of UD showed a strong positive correlation between the content of free oxyproline and the severity of dysmenorrhea (r=+0.86±0.25, p<0.02). In the group of girls with JD and CTD compared to the control group (χ2=4,302, p<0.03, OR≥4.71), a significant increase in the homozygous TT genotype was noted. The presence of COL1A1 and G2046T genes in the presence of criteria for undifferentiated connective tissue dysplasia in Uzbek women is a risk factor for the development of juvenile dysmenorrhea.