Influence of Adding Bergamot Essential Oil Against Staphylococcus Aureus Activity and Some Properties of Dental Stone

Abstract

An extremely critical field in dentistry to be investigated is the cross-contamination between dental patients and dental health care personnel regarding which dental cast is considered as a medium. Many researchers suggested the disinfection of dental stone by incorporating a certain disinfectant in the mixing liquid to gain antimicrobial activity while preserving acceptable properties of the material.This study was aimed to evaluate the antibacterial activity of bergamot essential oil incorporated in mixing liquid of type III dental stone against S.aureusand to evaluate the compressive strength and reproduction of details properties of the incorporated stone material. Methods:A total of 90 specimens, 30 specimens for each test with dimensions of 5 mm in diameter and 3 mm in thickness for antibacterial disk susceptibility test, 20 mm in diameter and 40 mm height for compressive strength test and 30 mm in diameter and 20 mm height for reproduction of details test  were prepared by addition of various percentages of BEO (8% and 10% by volume) and 0% as a control group. After isolation and identification of S. aureus, antibacterial tests were carried out to determine the minimum inhibitory concentration of the essential oil by broth micro dilution method, followed by both direct colony count and antibacterial disk susceptibility tests. Dental stone specimens were tested for compressive strength after 1 hour from the start of the mix. Regarding reproduction of details quality, specimens were evaluated by optical microscope. Results: Antibacterial tests revealed that a concentration of 8% is effective against S. aureus. The essential oil increased the compressive strength of dental stone and had no adverse effect on the reproduction of details quality. Conclusion: Bergamot essential oil could act as a potent antibacterial agent against S. aureus to obtain a disinfected dental cast with improved compressive strength and unaffected reproduction of details quality.