Morphological and Molecular Characterization of Proteus mirabilis Isolated from Different Clinical Samples in Najaf province

Abstract

The study included two main parts ,the first was the bacterial diagnosis based on relied diagnostic procedures .Second part were genetic study to virulence factors to common bacteria .A total of 148 samples were collected from different sources of patients that including (urine , vagina swab ,sputum ,tonsil) and from both sexes  during  a period  from August 2020 to November 2020.

These patients ranged from 1-69 years.(85) of them were females and (48)were males .The result indicated ,the most age group were more susceptible to infected (20_29) year from other age group, represented 75 patients with a percentage of (56.3%), among this group females were more susceptible with percentage (63.9%) compared to males (36%).

      The result indicated 133 samples (89.86%) gave  positive result , while 15 samples (10.13%) was Negative for culture bacteria , primary identification was depended on biochemical tests .Finally  identification with Vitek 2 system was done .The results demonstrate 42 isolate  E.coli ,31 isolate  P.mirabilis ,25 isolate K.pneumoniae , 21 isolate staphylococcus spp  ,7 isolate streptococcus spp,3 isolate pseudomonas spp and 4 isolate other bacteria .All isolates (100%) of E.coli and K.pneumoniae were able to produce capsule and biofilm .

     Regarding antibiotic susceptibility testing P. mirabilis isolates were resistant to Penicillin and sensitive to Amikacin .

The result indicated allP .mirabilis ,contain motA and motB genes contain motA which play role in the rotary motor of flagella with percentage 100%.