Extraction And Characterization of Type-I Collagen and Its Potential Application as Biopolimeric Films Loaded With Biosynthesized Silver Nanoparticles For Tissue Engineering

Abstract

Collagen has attracted great interest as a biomaterial in medical and tissue engineering applications. Also, the use of silver nanoparticles (AgNPs) as an antimicrobial in the field of biomedical has been of great interest in recent years. The present study aims to extract type-I collagen and to prepare biopolimeric films loaded with biosynthesized AgNPs for their potential application in tissue engineering. Collagen was extracted from the rat tail tendons using acid soluble collagen method and then characterized by SDS-PAGE, SEM, and FTIR analyzes. In addition, the stable AgNPs were synthesized by a green approach using aqueous extract of Achyranthus aspera leaves and characterized by UV-Vis spectroscopy, TEM and EDAX analyzes. Further, the thin biopolimeric films were prepared by incorporating collagen and AgNPs into polyvinyl alcohol (PVA), where the preparation method had no chemical reductant and crosslinking agent endowing high biocompatibility of the films. The AgNPs alone and PVA/Collagen/AgNPs biopolimeric films showed effective antibacterial activity against tested pathogenic bacterial strains. The cytotoxicity effect of collagen and AgNPs were tested against Vero cell line by MTT assay. The PVA/Collagen/AgNPs biopolimeric film showed significant In vitro wound scratch healing activity with 84.8% of wound closure.