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Breast cancer is the most common lethal cancer in women and the second most common among women worldwide. chemical carcinogen which interacts with membrane lipids, proteins, DNA and consequently induces free radical production. Cancer cell show various degree of differentiation, and there, normally is an inverse relation between the degree of cell differentiation and clinical aggressiveness of a cancer. To evaluate the antioxidants like both enzymatic and non-enzymatic levels in control and breast cancer patients, the Vitamin D in control and Breast cancer patients. Enzymic and nonenzymic antioxidants were examined. The activities of marker enzymes to confirm Breast cancer and the activity of Vitamin D were analyzed. The level of Vitamin D was found to be decreased in breast cancer patients. A decrease in the levels of enzymic antioxidants such as SOD, CAT, GPx, GR and nonenzymic antioxidants such as glutathione, vitamin C and vitamin E were observed in breast cancer patients. Carcino embryonic antigen is a marker of breast cancer were elevated in breast cancer patients.