Phylogenetic Tree Analysis of Klebsiella pneumoniae Isolated from Bat Myotis emarginatus based on 16srRNA Prokaryote Sequence Alignment

Abstract

Objective: Bats species are considered a reservoir of many pathogens which deemed important to human and animal health. However, there are studies was done on Klebsiella pneumoniae (K. pneumoniae) in these animals are very rare. The present study aimed to screen for the presence of K. pneumonia in bat species Myotis emarginatus and phylogenetic identification based on 16srRNA prokaryote gene Sequence alignment. Methods:K. pneumoniae selected from different organs of insectivory bat Myotis emarginatus (spleen, liver, kidney, lung, stomach, and intestines). K. pneumoniae recognized by cultures media and biochemical test than DNA extracted and submitted to PCR for amplification of 16srRNA prokaryote gene and phylogenetic analysis for one isolate performed depend on 16srRNA nucleotide sequences which searched in GenBank by using the BLAST. Results: Most bats harbor K. pneumoniae in the lung (20/30 (67%)) and the kidney (18/30 (60%)) whereas the less frequency of K. pneumoniae appeared in the spleen (5/30 (17%)).Nucleotide sequences of 16SrRNA geneobtained from K. pneumoniae isolated from batMyotis emarginatus were used to generate a phylogenetic tree based on the NCBI-GenBank database. GenBank number codes of these isolates are MK426995. In Conclusion:K. pneumoniae isolated from different tissues of bat Myotis emarginatus and the phylogenetic analysis of K. pneumoniae in NCBI-GenBank depend on 16S rRNA genea good option to identification and detection comparable strain of K. pneumoniae worldwide