The Repairing Effect of Γ-Oryzanol on the Ovarian Histological Structure of Transfluthrin-Exposed Female Rats (Rattus Norvegicus) from Endocrine-Disrupting Chemicals (EDCS)

Abstract

Rice bran contains many bioactive compounds of γ-Oryzanol.  We must reduce the use of mosquito repellent due to its impact on reproduction. One of the active compounds in mosquito repellent is pyrethroid derivatives known as transfluthrin, which belongs to the Endocrine-Disrupting Chemicals (EDCs). Therefore, the purpose of this study is to analyze the effect of γ-Oryzanol administration on repairing the ovarian histological structure from EDCs in transfluthrin-exposed female rats. This study used Foxo3a expression and TNF-α concentration as parameters. Moreover, the study went through the following stages. (1) In silico method consisted of (a) the effect of γ-Oryzanol on EDCs and (b) the effect of γ-Oryzanol on reproductive function through TNF-α concentration and Foxo3a expression. (2) In vivo method included a post-test only control group design applied using Wistar females divided into 4 (four) groups. The groups were exposed to one push of 21.3% transfluthrin for 6 hours and were administered with γ-Oryzanol for 28 days. The analysis was done with Kruskal Wallis’s statistical analysis and Mann Whitney’s posthoc with IBM SPSS Statistics 25 software. Findings confirm the following. (1) There is an effect of γ-Oryzanol on EDCs through adenylate cyclase activation (HTR1A and HTR1B, serotonin receptor genes). Y-oryzanol also has an inflammatory activity and nitric oxide scavengers, which influence TNF-α concentration and Foxo3a expression through APP and AKT1. (2) γ-Oryzanol stimulates follicle growth and effectively reduces the number of follicular abnormalities. (3) γ-Oryzanol is found to have the potential of maintaining a normal estrous cycle by controlling Foxo3a expression and TNF-α concentration. From these results, it can be concluded that γ-Oryzanol is effective to counter EDCs effects, reduce follicular abnormalities, and reduce Foxo3a expression and TNF-α concentration.