The Ethyl Acetate Extract of Faloak Leaves (Sterculiaquadrifida R.Br) inhibits Human Cervical Cancer Cells Proliferation and induce Cell Death

Abstract

Cervical cancer is the third most common form of cancer in women around the world. Medicinal plants have been used to cure diseases for a long time. Ethyl acetate extract of faloak leaves (EFL) (Sterculiaquadrifida R.Br) contains flavonoid, tannins, saponins, and terpenoids that are potentially good as anti-cancer drugs. This study aims to investigate the effect of EFL on HeLa cells of cervical cancer through treatment of a combination of faloak leaves extract and chemotherapy agent paclitaxel (Pac). Cells were treated without Pac of control group, combination treatment group of EFL 25 μg/mL, 50 μg/mL, 100 μg/mL and 5 μg/mL Pac and a single dose of 100 μg/mL EFL. Inhibition of proliferation activity was measured by 3-(4,5 –dimethylthiazol –2–yl )-2,5 diphenyltetrazolium bromide (MTT) assay and HeLa cell death was observed with blue trypan. The anticancer activity compounds fromthis results shown that EFL with or without combination effectively inhibit the proliferation ofHeLa cells. This extract induce HeLa cell death- dependent on the combination of (25, 50, 100 μg/mL) with 5 μg/mL Pac. It can be concluded that ethyl acetate extract of faloak leaves can inhibit proliferation and increase HeLa cell death.