Evaluation of antivenom potential of NataKushtadi Yoga against cobra venom, in-vitro study

Abstract

Background:The Indian Cobra (Najanaja) is considered very dangerous snake and it is commonly associated with high human mortality rate in India. Due to rapid action of neurotoxins it produces systemic poisoning which causes respiratory paralysis, the major cause of death. Cardio respiratory support and Anti Snake Venom (ASV) are the most important and effective tools for snake bite treatment. ASV has many side effects like anaphylaxis,pyrogen reactions and serum sickness.To avoid the above stated risk due to ASV treatment there is anurgent need to develop and search the affordable and suitable antidote as alternative treatment. The Indian Medicinal Plants extracts has veryplentiful source of pharmacologically active compounds and theirextracts has property to act against snake venom.The present study has been planned with objective to examine the therapeutic potential of Natkusthadi yoga by analytical study and as an antidote to neutralize the adverse action of snake venom by acetylcholinesterase and Phospholipase A2 (PLA2)inhibition activity.

Aim:-  Assessment of Natakushtadi Yoga for anti-venom enzymatic activities against cobra venom.

Material &method:-The Acetylcholinesteraseinhibition activity will be measured by following Ellman et al. method and Phospholipase A2 (PLA2)inhibition activity will be measure using an indirect hemolytic assay on Agarose-egg yolk gel plate by following BrindhaDurairaj Method.

Result: - The water extract of Natakushtadi Yogawill manifest a notable inhibitory effect on Acetylcholinesterase and Phospholipase A2 (PLA2) enzymes found in cobra venom.