Molecular Diagnosis of S. Mutans Isolated from Dental Caries in Thi-Qar Province

Abstract

The aim of this study was to determine the prevalence of S. mutans isolates that isolated from patient mouths of the dental caries (n=100) in Nasseriha city through the period February to June,2020 at Thi-Qar province. The isolates were identified as S. mutans according to the culture methods and biochemical tests, and to analyze by the polymerase chain reaction (PCR) technique to diagnosis those isolates molecularly by the 16s RNA gene and detected two serotype C and K genes. The results of current study showed the occurrence of S. mutans were 24% from all collected samples. The identification of S. mutans by 16s RNA gene recoded that all isolates (100%) of S. mutans harbored this gene. Whereas the results of serotype C and K genes showed 4.166% only had serotype C gene, while entirely isolates of S. mutans no contain serotype K gene.