A Surveillance of Antimicrobial Resistance Pattern and Virulence Factor in Uropathogenicescherichia Coli

Abstract

 Escherichia coli (E.coli) is known to be the pathogen commonly isolated from those infected with urinary tract infections (UTIs). The aim of this study was to investigate the presence of E.coli virulence factor and antibiotics resistance pattern among clinical isolates from theurinary tract infections patient. 94 E.coliisolates from 166 UTI patients were taken as cases & 20 E.coliisolated from healthy individuals were taken as controls. Out of 94 isolates, 75(79.78%) were identified as UPEC in cases and 7(35%) were from 20 isolates in controls. Hemolysin production & cell surface hydrophobicity was found to be the major combination(36%) followed by serum resistance&hemolysinproduction (26.66%),serum resistance & cell surface hydrophobicity(22.66%). Most resistant drugs were Cotrimoxazole (65.95 %) followed byciprofloxacin (42.55%) &gatifloxacin(32.04%). Imipenem(92.56%) &amikacin(90.43%) were found to be highly sensitive drugs. Though CSH was major virulence factor among all, hemolysin production was detected predominantly in MDR strains(76.92%) as well as ESBL producers(51.28%). Interestingly also we observed that virulence factors production was inversely related to ESBL production. As most urovirulent strains express multiple virulence factors simultaneously, further studies on interaction of different virulence factors at molecular labelare necessary.