Characterization of Streptomyces Smyrnaeus Ati-92 Isolated from Soil Samples in Basrah City

Abstract

The present study aimed to isolate and identify local Streptomyces from different sites of Basrah city, Iraq. A total of 150 samples of (soil, cow feces, buffalo feces) were collected. However, thirty-one local isolate of promising Streptomyces spp. were obtained from soil, cow, and buffalo feces after cultivated on the Inorganic Salt Starch Agar ISP4, Depend on the morphological, microscopic, biochemical characteristics, and antimicrobial activity, the isolate S13 was found to be a member of the genus Streptomyces. Furthermore, genetic diagnoses were performed on the Streptomyces 13. The analysis of 16S rRNA gene sequence similarity and the phylogenetic tree showed that Streptomyces S13 isolate was closely related to Streptomyces smyrnaeus (99.51%), was deposited in the NCBI gene bank (Streptomyces) Ati-92 smyrnaeus with accession number (LC495904).