Covid -19 Virus Gene Distinction and Allelic Discrimination in Infected Patients in Babylon, Province

Abstract

The term coronavirus was first specified as a virus having spike-like projections, Coronaviruses (CoVs) are a group of related RNA viruses that cause diseases in both human and vertebrates. They can infect the respiratory, and gastrointestinal tract, hepatic, and central nervous system of human, livestock, birds, bat, mouse, and many other wild animals, which are enveloped viruses that possess large-sized single-strand RNA genomes. On December 29, 2019, the WHO officially named the novel disease as coronavirus disease 2019 (COVID-19). This virus can enter the human body by its Angiotensin-converting enzyme 2 (ACE2) receptors which are located in various organs such as the heart, lungs, kidneys, and digestive system, which is complementary in shape to the spike shape which allows successful attachment to occur and makes it easier for the virus to enter the target cells. From a group of probable cases in which that infected with SARS-COV-2 virus (Covid-19) taken the nasopharyngeal swab into viral transport Media (VTM) from 120 patients as studied group, all samples were chickened by using Direct Immunofluorescences antibody procedure provided by (Viro-Cell Company). The VTM containing Nasopharyngeal swab was taking to Real Time PCR, by Using Bioron– Real Time Kit, to evaluate the presence of gene differentiation of SARS -COV-2 as well as allelic discrimination through specific protocol provided with Bioron Kit on the Bio –Rad Thermo cycler system. The statistical analysis was done by using SPSS, ANOVA and Chi – Square.Gene distinction of SARS-CoV-2 with allelic discrimination by real time PCR. The presence of S gene mostly in first few days of infection while E gene present in the end time of infection over 1 -2 week of onset of infection as well as N gene but in less degree. The N gene has a higher level of stability and conservation, With high percent amino acid similarity and less mutations over time. The heterozygote of ( S and / E gene ) have higher percentage than other.